DUF4297 and HerA form abortosome to mediate bacterial immunity against phage infection. Dongmei Tang et al.
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Figure1. 1D Cell viability assessed for strains expressing EcDUF4297‒EcHerA, EcDUF4297 alone, or EcHerA alone by serial dilutions. 1G The nuclease and ATPase activities of DUF4297‒HerA. 1H Hailibu degrades phage genome, bacterial genome, and PCR product. 1J The in vivo nuclease activity of Hailibu system. Figure3. 3B Sequencing raw data of DNA degradation products by Hailibu abortosome. Figure S1. S1A. Degradation of genome DNA of P1-infected E. coli cells in the presence of EcHailibu system. S1B.The nuclease activity of EcHailibu under various ATP concentrations. S1C. The nuclease activity of EcHailibu with various adenosine nucleotides. S1D. Thin-layer chromatography analysis of EcHailibu defense system ATPase against ATP. Figure S3. S3A.The nuclease activity of AtHailibu. S3B. The nuclease and ATPase activities of AtHailibu. S3C. AtHailibu degrades phage genome, bacterial genome, and PCR product. S3D.The nuclease activity of AtHailibu under various ATP concentrations. S3E. The nuclease activity of AtHailibu with various adenosine nucleotides. S3G.Thin-layer chromatography analysis of the ATPase activity of AtHailibu.
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Sichuan Science and Technology Program
2024NSFTD0029
National Natural Science Foundation of China
32270761
Natural Science Foundation of Sichuan Province
24NSFSC3258
Postdoctoral Research Fund of West China Hospital, Sichuan University
2024HXBH125
China Postdoctoral Science Foundation
2024T170618
National Natural Science Foundation of China
32400125
China Postdoctoral Science Foundation
2022M712272