DUF4297 and HerA form abortosome to mediate bacterial immunity against phage infection. Dongmei Tang et al.

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Figure1. 1D Cell viability assessed for strains expressing EcDUF4297‒EcHerA, EcDUF4297 alone, or EcHerA alone by serial dilutions. 1G The nuclease and ATPase activities of DUF4297‒HerA. 1H Hailibu degrades phage genome, bacterial genome, and PCR product. 1J The in vivo nuclease activity of Hailibu system. Figure3. 3B Sequencing raw data of DNA degradation products by Hailibu abortosome. Figure S1. S1A. Degradation of genome DNA of P1-infected E. coli cells in the presence of EcHailibu system. S1B.The nuclease activity of EcHailibu under various ATP concentrations. S1C. The nuclease activity of EcHailibu with various adenosine nucleotides. S1D. Thin-layer chromatography analysis of EcHailibu defense system ATPase against ATP. Figure S3. S3A.The nuclease activity of AtHailibu. S3B. The nuclease and ATPase activities of AtHailibu. S3C. AtHailibu degrades phage genome, bacterial genome, and PCR product. S3D.The nuclease activity of AtHailibu under various ATP concentrations. S3E. The nuclease activity of AtHailibu with various adenosine nucleotides. S3G.Thin-layer chromatography analysis of the ATPase activity of AtHailibu.

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