DUF4297 and HerA form abortosome to mediate bacterial immunity against phage infection. Dongmei Tang et al.

Published: 26 November 2024| Version 2 | DOI: 10.17632/cg3jmcgh4w.2
Contributor:
冬梅

Description

Figure1. 1D Cell viability assessed for strains expressing EcDUF4297‒EcHerA, EcDUF4297 alone, or EcHerA alone by serial dilutions. 1G The nuclease and ATPase activities of DUF4297‒HerA. 1H Hailibu degrades phage genome, bacterial genome, and PCR product. 1J The in vivo nuclease activity of Hailibu system. Figure3. 3B Sequencing raw data of DNA degradation products by Hailibu abortosome. Figure S1. S1A. Degradation of genome DNA of P1-infected E. coli cells in the presence of EcHailibu system. S1B.The nuclease activity of EcHailibu under various ATP concentrations. S1C. The nuclease activity of EcHailibu with various adenosine nucleotides. S1D. Thin-layer chromatography analysis of EcHailibu defense system ATPase against ATP. Figure S3. S3A.The nuclease activity of AtHailibu. S3B. The nuclease and ATPase activities of AtHailibu. S3C. AtHailibu degrades phage genome, bacterial genome, and PCR product. S3D.The nuclease activity of AtHailibu under various ATP concentrations. S3E. The nuclease activity of AtHailibu with various adenosine nucleotides. S3G.Thin-layer chromatography analysis of the ATPase activity of AtHailibu.

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Institutions

Sichuan University

Categories

Thin-Layer Chromatography, DNA Sequencing, Gel

Funding

Sichuan Science and Technology Program

2024NSFTD0029

National Natural Science Foundation of China

32270761

Natural Science Foundation of Sichuan Province

24NSFSC3258

Postdoctoral Research Fund of West China Hospital, Sichuan University

2024HXBH125

China Postdoctoral Science Foundation

2024T170618

National Natural Science Foundation of China

32400125

China Postdoctoral Science Foundation

2022M712272

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