The polysaccharides of chinese actinidia root
Description
To investigate the therapeutic effects of the polysaccharides of chinese actinidia root (PCAR) on acute liver injury and its potential as an ingredient in drugs and nutraceuticals. Microwave-assisted extraction technology combined with Box-Behnken model combined with the three kinds of artificial neural networks was used to optimize PCAR extraction process. Characterize the structure and composition of PCAR. To prevent the degradation of PCAR by the gastrointestinal environment after oral administration, PCAR-loaded liposomes were prepared. The in vivo efficacy of PCAR-loaded liposomes was evaluated by three acute liver injury animal models prepared according to different mechanisms. The best yield of PCAR was 4.49 %, and the purity reached up to 86.53 % by optimizing the microwave parameters using Box-Behnken model combined with the three kinds of artificial neural networks. PCAR with ɑ-glycosidic and β-glycosidic bonds is mainly composed of galactose (60.85 %) and xylose (14.45 %), with a triple helix structure and a very low content of proteins and nucleic acids. The PCAR-loaded liposome obtained had a size about 340 nm with a polydispersity index 0.234. The entrapment efficiency was 70.12 % and the drug loading was 1.24 %. Liposomes can be fully released in the gastrointestinal environment within 12 hours and have long-term stability at 4°C. The therapeutic effect of PCAR liposomes on acute liver injury was confirmed by three animal models of acute liver injury.Our study reveals how artificial neural network uncover complex links between PCAR extraction process, supporting the potential of PCAR for acute liver injury treatment.