A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging by Falkovich et al.

Published: 21 April 2023| Version 1 | DOI: 10.17632/cjyzby7z82.1


The attached dataset consists of measurements of individual (per-synapse) fluorescence intensity integrals of 11 proteins in ~1 million synapses, across 250 wells and 19 treatment groups. The dataset was obtained by combining automatic CellProfiler quantification of images from four experiments (each protein in each experiment normalized by mean intensity of that protein in untreated wells), with rigid manually set threshold levels. Associated data, including raw and processed images and CellProfiler output with other parameters is available upon request. Associated with paper: 10.1016/j.celrep.2023.112430 and software: 10.5281/zenodo.7753915


Steps to reproduce

CellProfiler pipelines and R scripts to reproduce these synaptic measurements from PRISM images are available on github.com/lcbb


Image Quantification