CD21hi and CD21lo Memory B cell bulk RNAseq gene transcript FPKM values

Published: 20-06-2019| Version 1 | DOI: 10.17632/cnjsg3yggy.1
Contributors:
Sarah Andrews,
Adrian McDermott,
Sam Darko,
Jianfei Hu,
Daniel Douek

Description

CD21hiCD19int and CD21loCD19hi IgG+ memory B cells were sorted from frozen PBMCS from four healthy adults. Sorted cells were frozen in RNAzol (Molecular Research Center) and RNA extracted according to the manufacturer’s instructions. mRNA libraries were constructed as described (Sandler et al., 2014) and sequenced using the Illumina TruSeq PE paired-end cluster kit on a HiSeq 2000. Trimmomatic (version 0.22) (Bolger et al., 2014) was used to remove adapters and low-quality bases. The trimmed paired-end reads were mapped to the reference human genome (Hg19) using Tophat (version 2.0.8) with a reference annotation (Ensembl“Homo_sapiens.GRCh37.74.gtf”). Samples with low map rates were discarded. Transcript abundance was determined with Cufflinks (version 2.1.1). This table lists FPKM values for all genes in each of the two sorted populations from each of the four individuals.

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