in vitro growth detection of intestinal bacteria

Description

This study proposed a novel growth detection method for anaerobic microbe. By testing the effects of fructose and procyanidin on Clostridium butyricum, Bifidobacterium longum and Enterococcus faecalis, it was shown that this method had a good effect for anaerobic culture. Furthermore, through the multi-day transfer culture of mixed intestinal bacteria, it showed that the method had good repeatability and reliability, which was suitable for comparing and screening the effects of drugs on intestinal bacteria. The effects of FOS and OPC on the growth of three strains were also detected. The results showed that the presence of FOS could promote the rapid growth of C. butyricum in the logarithmic phase, but the high concentration of FOS could inhibit the growth of C. butyricum in the stationary phase. Similarly ( Fig. 2A ). OPC had little effect on the growth of C. butyricum. For B. longum ( Fig. 2B ), in the rapid growth stage, both high and low concentrations of FOS or OPC have a certain inhibitory effect on its growth, but in the stationary phase, only high concentrations of OPC have a certain inhibitory effect on its growth. For E. faecalis ( Fig. 2C ), in the rapid growth stage, both high and low concentrations of FOS or OPC had little effect on its growth, but in the stationary phase, low concentrations of FOS had a certain growth promoting effect. The effects of P. fermentans 15B1 and P. fermentans 323 strains on the diversity of intestinal bacteria were similar (Fig. 4). The analysis to the main 10 genera showed that the overall change of intestinal microbiota induced by P. fermentans 15B1 and P. fermentans 323 were similar, but there were some significant differences compared with the control ( Fig 5A ). The changes of Bifidobacterium, Collinsella, Escherichia and Streptococcus had the highest abundance within 4 days ( Fig.5B )

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