Venom Sampling via Trivial Centrifugation

Published: 5 March 2018| Version 1 | DOI: 10.17632/cpnscw2gkc.1
Contributors:
Eduardo Fox,
Meng Xu,
Lei Wang,
Li Chen,
Yongyue Lu

Description

This is the supporting raw data and information behind the published paper "Speedy milking of fresh venom from Aculeate hymenopterans" by Fox et al. (2018), available at: https://www.sciencedirect.com/science/article/pii/S0041010118300990 It includes: - Draft version of the manuscript prior to submission; - Peer review details; - Raw chromatograms obtained for centrifuge-milked venoms of fire ants, two species of bees, one species of Asian hornet; - Raw chromatograms of internal and calibration standards; - Images and details on the identification of utilised insects; - Pictures obtained for the venoms extracted and utilised structures. Disclaimer: The presented method for venom extraction is extremely simple, and can be extended to other poisonous animal through simple adaptations. Such secretions can be highly dangerous. Responsible use of this technology is strongly recommended. Venom obtained via such method is to be used for legal, surveilled scientific purposes **only**.

Files

Steps to reproduce

-Isolate living venom apparatuses of arthropods; -fit glass wool or a piece of metal mesh into a glass vial insert as to form a basket-shaped stopper prior to the bottom of the tube; - carefully relocate the venom-containing tissues into the basket-containing insert; - add the glass insert into a trivial centrifuge tube; - organise the tubes in a trivial centrifuge as for normal operation; - centrifuge the tubes for short 30-60s cycles of 'mild' centrifugation (this can be case-specific) ; - open the centrifuge and gently move the venom apparatuses around the basket to change their orientation for pressuring. - Centrifuge again. After 5-10 cycles find the collected venom at the bottom of the glass insert, ready for bioassays.

Institutions

Institute of Zoology Chinese Academy of Sciences, South China Agricultural University

Categories

Natural Sciences, Zoology, Entomology, Chromatography, Chemical Ecology, Gas Chromatography, Sample Extraction, Animal Science, Medical Biology, Enzyme Secretion, Bioassay, Analytical Method, Toxins, Poison, Centrifugation, Poisonous Residues, Ant, Bug, Toxinology

License