Venom Sampling via Trivial Centrifugation

Published: 5 Mar 2018 | Version 1 | DOI: 10.17632/cpnscw2gkc.1
Contributor(s):
  • Eduardo Fox,
    Unspecified
    South China Agricultural University
    Devised the method, reared the insects, dissected and handled samples, calibrated and operated GC-MS, interpreted the results, wrote scientific papers, organized and deposited the present dataset.
  • Meng Xu,
    Meng Xu
    CAS
    Retrieved and injected chemical standards
  • Lei Wang,
    Lei Wang
    South China Agricultural University
    Provided insights, took care of colonies, managed GC-MS
  • Li Chen,
    Li Chen
    Institute of Zoology Chinese Academy of Sciences
    Purchased standards and provided technical insights
  • Yongyue Lu
    Yongyue Lu
    South China Agricultural University
    Signed some paper and funded a fire ant collection trip

Description of this data

This is the supporting raw data and information behind the published paper "Speedy milking of fresh venom from Aculeate hymenopterans" by Fox et al. (2018), available at:
https://www.sciencedirect.com/science/article/pii/S0041010118300990

It includes:

  • Draft version of the manuscript prior to submission;
  • Peer review details;
  • Raw chromatograms obtained for centrifuge-milked venoms of fire ants, two species of bees, one species of Asian hornet;
  • Raw chromatograms of internal and calibration standards;
  • Images and details on the identification of utilised insects;
  • Pictures obtained for the venoms extracted and utilised structures.

Disclaimer:
The presented method for venom extraction is extremely simple, and can be extended to other poisonous animal through simple adaptations. Such secretions can be highly dangerous. Responsible use of this technology is strongly recommended. Venom obtained via such method is to be used for legal, surveilled scientific purposes **only**.

Experiment data files

  • Original chromatograms
    Cite
    • Calibration
      Cite
    • Injected Samples
      Cite
  • Original Pictures
    Cite
    • calibration samples
      Cite
    • Venoms
      Cite
  • Submission Details
    Cite
    • Prior to Submission
      Cite

Steps to reproduce

-Isolate living venom apparatuses of arthropods;
-fit glass wool or a piece of metal mesh into a glass vial insert as to form a basket-shaped stopper prior to the bottom of the tube;

  • carefully relocate the venom-containing tissues into the basket-containing insert;
  • add the glass insert into a trivial centrifuge tube;
  • organise the tubes in a trivial centrifuge as for normal operation;
  • centrifuge the tubes for short 30-60s cycles of 'mild' centrifugation (this can be case-specific) ;
  • open the centrifuge and gently move the venom apparatuses around the basket to change their orientation for pressuring.
  • Centrifuge again.
    After 5-10 cycles find the collected venom at the bottom of the glass insert, ready for bioassays.

Related links

This data is associated with the following publication:

Speedy milking of fresh venom from aculeate hymenopterans

Published in: Toxicon

Latest version

  • Version 1

    2018-03-05

    Published: 2018-03-05

    DOI: 10.17632/cpnscw2gkc.1

    Cite this dataset

    Fox, Eduardo; Xu, Meng; Wang, Lei; Chen, Li; Lu, Yongyue (2018), “Venom Sampling via Trivial Centrifugation”, Mendeley Data, v1 http://dx.doi.org/10.17632/cpnscw2gkc.1

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Institutions

Institute of Zoology Chinese Academy of Sciences, South China Agricultural University

Categories

Natural Sciences, Zoology, Entomology, Chromatography, Chemical Ecology, Gas Chromatography, Sample Extraction, Animal Science, Medical Biology, Enzyme Secretion, Bioassay, Analytical Method, Toxins, Poison, Centrifugation, Poisonous Residues, Ant, Bug, Toxinology

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

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