In vivo tracking of from the fruiting body of Cordyceps militaris extracts and protective effects against CCl4-induced acute liver injury in mice

Published: 5 September 2023| Version 1 | DOI: 10.17632/crhfg9j47g.1
lin lan


Cordyceps militaris is a well-known medicinal and food plant that has attracted academic attention for its polysaccharides with a wide range of physiological activities and health benefits. The active polysaccharide CM-1 was obtained through hot water immersion and column chromatography to separate the pure precip-itate, and its structural characteristics were analyzed using spectral and chemical methods. The antioxidant effect of CM-1 was demonstrated through the DPPH free radical scavenging test and the determination of total antioxidant capacity. Furthermore, iodine substitution absorption utilization was analyzed, and the hepatoprotective effect of CM-1 on CCl4-induced injury in mice was evaluated in vivo. The results showed that CM-1 had an absorption rate of 5.71% in the blood, with the highest content in the liver being 1.24 mg. CM-1 exhibited a positive protective effect on CCl4-induced acute liver injury in mice, reducing serum as-partate aminotransferase (AST) and alanine aminotransferase (ALT) levels, and improving the expression levels of GSH-Px, SOD, and CAT in CCl4-induced liver injury. Additionally, CM-1 treatment helped alleviate the expression of MDA and protected mice from acute liver injury induced by CCl4. These findings suggest that CM-1 has significant potential as a dietary supplement for the prevention and treatment of liver injury.



Xihua University


Liver Protective Agent, Extraction Method, Bioactivity of Polysaccharides