The source file for unprocessed immuno-blots for MOLECULAR-CELL-D-17-01671

Published: 12-04-2018| Version 1 | DOI: 10.17632/cszpvms38z.1
Xiaoling Li,
He Huang,
Shuang Tang,
Ming Ji,
Zhanyun Tang,
Yingming Zhao,
Robert Roeder


In this study, we investigated the role of p300 in mediating a novel protein lysine acylation, lysine 2-hydroxyisobutyration (Khib), as well as its functional impact on cellular glucose metabolism. Using the immuno-blotting method, we show that p300 functions as a lysine 2-hyroxyisobutyryltransferase to modify both histones and glycolytic enzymes, activating gene transcription and promoting glycolysis.