Details of primers for target and reference genes analyzed by qPCR
Published: 10 March 2023| Version 2 | DOI: 10.17632/ct76pt4z68.2
Details of primers for target and reference genes analyzed by qPCR used for cotyledons expression. Primer designs were obtained from Rotta et al. (2015) and from GenBank for the following targets: 18S ribosomal RNA, vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor B (VEGFB), insulin-like growth factor 1 receptor (IGFR1), insulin-like growth factor 2 receptor (IGFR2), guanilate cyclase 1 soluble subunit beta (GUCY1B3), hypoxia inducible factor 1-alpha (HIFA), splicing factor 3a subunit 1 (SF3A1), fibroblast growth factor 2 (FGF2), angiopoietin 2 (ANGPT2), and nitric oxide synthase (NOS3).
Universidade Federal de Vicosa
Real-Time Polymerase Chain Reaction