Bioactivities of Polyphenols, Polysaccharides, and Oligosaccharides Derived from Two Wild West African Ganoderma Species
Description
This dataset supports the findings of the article titled "Bioactivities of Polyphenols, Polysaccharides, and Oligosaccharides Derived from Two Wild West African Ganoderma Species". It contains raw and processed data for antioxidant (DPPH), antibacterial (MIC and inhibition percentages), and anticancer (XTT cell viability) assays of extracts from Ganoderma enigmaticum and Ganoderma mbrekobenum. Supplementary data include total phenolic and carbohydrate content, extraction yields, and sequencing data for species identification using the ITS rDNA region. The dataset may be useful for researchers working on medicinal mushrooms, drug discovery, and natural product pharmacology.
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This dataset was generated during a study investigating the bioactivities of polyphenolic, polysaccharide, and oligosaccharide fractions from two newly described West African Ganoderma species—G. enigmaticum and G. mbrekobenum. The following procedures were employed: 1. Sample Collection and Molecular Identification • Fruiting bodies of both species were collected from decaying wood in Lagos Nigeria • Dried specimens were pulverized for DNA extraction using the Norgen Fungal/Bacterial DNA Isolation Kit. • The ITS1-5.8S-ITS2 rDNA region was amplified by PCR using primers ITS1 and ITS4. • Amplicons were sequenced using the Sanger method. • Raw sequences were edited and assembled using Geneious Prime, and species identity was confirmed via BLAST searches (NCBI). 2. Extraction of Bioactive Fractions • Polyphenols were extracted using 70% acidified methanol (HCl-acidified), followed by rotary evaporation and filtration • Polysaccharides were obtained via hot water extraction, ethanol precipitation, and freeze-drying • Oligosaccharides were isolated by mild acid hydrolysis (1% formic acid) of polysaccharides and purified by ethanol fractionation. 3. Quantification of Phenolics and Carbohydrates • Total phenolic content was quantified by the Folin–Ciocalteu method using gallic acid as standard. • Total carbohydrate content was determined using the Phenol-sulfuric method with glucose as standard. • Absorbance was measured at appropriate wavelengths using the BioTek Multiskan Microplate Reader (Bio Rad). 4. Antioxidant Activity Assay • DPPH radical scavenging assay was conducted to evaluate antioxidant potential across various concentrations (25–100 µg/ml). • Absorbance was read at 517 nm using the microplate reader, and inhibition percentages were calculated. 5. Antibacterial Activity Assay • Broth microdilution assay was used to assess antibacterial activity against: o Escherichia coli O157:H7 (ATCC BAA-3162) o Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 700968) • Half maximal inhibitory concentration (IC50) was computed using percentage inhibition of different concentrations of the extracts with the GraphPad Prism software 6. Anticancer Assay • The XTT Cell Proliferation Assay Kit (ATCC® 30-1011K™) was used on: o HepG2 (liver cancer) o HCT116 (colorectal cancer) o MDA-MB-231 (triple-negative breast cancer) • Extracts were tested at concentrations between 25–100 µg/ml. • IC₅₀ values were calculated from dose-response curves. 7. Software and Data Analysis • Sequence editing and alignment: Geneious Prime • Sequence validation: NCBI BLAST • Graphing and statistics: GraphPad Prism 10 and Microsoft Excel