Dendritic cell Differentiation: A case of rapid transcriptome change and unreliable data normalization

Published: 2 May 2023| Version 1 | DOI: 10.17632/cwfxh3n945.1
Sanjesh Saini, Nishtha Agrawal, Nilanshu Manocha, Archana Saini, Madhu Khanna


The present study evaluated the expression of eleven commonly used housekeeping during the differentiation of bone marrow derived dendritic cells of mice from the precursor cells. The expression of these genes was studied over the period of seven days during the differentiation process. The PCR based expression data of all the genes was also analyzed by gNorm to determine the most stable reference gene. Based upon the M value V value data of gNorm; minimum two reference genes with lowest M value are sufficient for data normalization in current study.



Molecular Biology, Microbiology, Real-Time Polymerase Chain Reaction, Clinical Virology