Data S1 - Nanostring

Published: 25-06-2020| Version 1 | DOI: 10.17632/cxs7xwjpfj.1
Contributor:
Olivier Terrier

Description

Innate immune transcriptional signature during the time course of SARS-COV-2 infection in Human Airway Epithelia (MOI 0.1 24, 48 72hpi). After on-column mRNA extraction, gene expression levels were evaluated using two customized NanoString nCounter Gene Expression Panels: “immune response” (96 genes) and “type III IFNs” (12 genes). Data processing and normalization were performed with nSolver analysis software (version 4.0, NanoString technologies) and results are expressed in fold change induction compared to the mock condition. Heatmap and Principal Component Analysis (PCA) were produced using Genomics Suite 7 (Partek, St Louis, MO, USA). After on-columns mRNA extraction, expression was evaluated using two different gene panels (composed of 96 and 12 genes respectively) using the Nanostring technology. Data treatment and normalization ware performed with nSolver analysis software (version 4.0, NanoString technologies). Results are expressed in counts and fold change induction compared to the mock condition.

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