Data Set on the effect of oleic acid on the Reproductive Immuno-endocrine Regulation of Female Wistar Rats on an Unrestricted Diet
Description
We sought to ascertain if oleic acid, when administered to female Wistar rats on an uncontrolled or unrestricted diet, could alter the levels of immune white blood cells and reproductive endocrine indices. The data were obtained from female Wistar rats (n=18, 10- 12 weeks old, 151 ± 24 g) on an unrestricted diet and treated with different oleic acid doses. The effect of oleic acid (364525-IL Sigma-Aldrich, USA) at 0, 500 and 1000 mg/kg body weight was investigated on the full-blood count (WBC and RBC parameters), and reproductive endocrine indices (reproductive hormones, reproductive organ weights and oestrous cyclicity) of female Wistar rats treated for three cycles (15 days). The results indicated that oleic acid treatment induced increased body weight gain and relative reproductive tract weight in the rats. However, it did not affect Wistar rats reproductive hormone levels, oestrous cycle and RBC parameters. Notably, oleic acid altered the white blood cell parameters in the rats, specifically reducing the lymphocyte population of white blood cells and increasing the Mid- cells (Basophils, eosinophils, monocytes, and mast cells) and neutrophils. [Data 1] Body weights of the female Wistar rats before treatment commenced, and after treatment, and the increases in body weights were measured and presented. [Data 2] The organ weights (paired ovaries and reproductive tract) were presented relative to the body weights as g/100 g body weight. [Data 3] The rats were classified into four oestrous stages; proestrus, oestrus, metoestrus, and dioestrus. The stages were assigned numbers 1, 2, 3, and 4, respectively. [Data 4] The blood of five rats from each group was analyzed for its full blood count (Red blood cells, white blood cells, and plateletes). [Data 5] The rat's serum was analyzed for oestrogen, progesterone, and LH levels.
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1. Initial body weight was the weight of the rats just before treatment started. The final body weight was the weight of the rats at euthanasia, after 15 days of treatment. The body weight gain was the difference between the initial and final body weights. 2. After treatment, the rats were euthanised by cervical dislocation. The reproductive organs were harvested, blotted on paper to clean off blood, debrided of connective tissue, and weighed. 3. Vaginal smears were collected before treatment began and at the end of treatment; The vagina of the rats was flushed with 50 µl of distilled water and the suspension was aspirated and deposited on clean slides, allowed to dry, fixed in absolute ethanol, and stained with Giemsa stain. The slides were viewed under the microscope at X10 and 40 magnifications and the rats’ oestrous stages were classified as proestrus, oestrus, metoestrus and dioestrus when the smear was predominantly round nucleated cells (proestrus), superficial polygonal cornified epithelial cells (oestrus), an equal proportion of all cells (metoestrus), and predominantly neutrophils (dioestrus). 4. Blood was collected from slightly anaesthetised rats and collected through a capillary tube from the median cantus of the eyes into EDTA vacutainer tubes and plain vacutainer tubes. The blood in EDTA vacutainer tubes was processed for a complete hemogram using MC Jefferson LE046D 3-part Automated haemoanalyzer. 5. The blood in plain vacutainer tubes was centrifuged at 1000 g and the serum was subjected to a chemiluminescence immunoassay for oestrogen, progesterone, and LH using Maglumi 800 Chemiluminescence Analyzer.