Synovial tissues histology from patients with end-stage osteoarthritis, soft tissue and traumatic injuries of the knee
Description
Cartilage degeneration is the hallmark of osteoarthritis (OA). The research hypothesis investigated was that the inflammatory response of synovium to different insults would vary both with the insult and with patient characteristics. We sought to determine which elements of synovial reactivity were linked to cartilage debris in OA patients. The data shows histopathology microimages of haematoxylin and eosin (HE)-stained knee synovial tissues from osteoarthritic total knee replacement and non-osteoarthritic soft tissue and trauma injury patients. Knee medial gutter synovial tissue samples were obtained from a cohort of 15 endstage primary osteoarthritis, and 17 patients with no clinical or radiological evidence of arthritis, 9 ligament tears and 8 tibial plateau fractures. Images show evidence of varying degrees of synovitis in the three different pathology groups. In addition, features such as cartilage debris and focal aggregations of inflammatory cells were noted. The Mendeley data repository contains 32 individual patient folders containing the H&E-stained synovial tissue section images with identification grids and location maps. Each H&E folder holds a complete tissue section image labelled as HE_sample id_full, an image grids trail map labelled as HE_sample id_map, together with sequentially numbered image grids. Evidence of cartilage debris and inflammatory cells infiltration were most notable in the OA group. Individual folder IDs can be linked to an anonymised set of individual patient’s data in the data in brief submission. Linked anonymised clinical data includes patient’s pathology, gender, age, body mass index (BMI), duration of symptoms, blood pressure, leukocytes count, C-reactive protein concentration, S100A8A9 protein level, ASA grade, cumulative illness rating scale (CIRS), WOMAC osteoarthritis index score, SF-12 physical component score and SF-12 mental component score. The holistic data can therefore inform of potential relationships between patient well-being, knee x-ray data, function and synovial tissue features. The data were acquired following informed consent (Medical ethics approval reference: 15/NW/0661). All patients underwent knee surgery at the Liverpool University Hospitals NHS Foundation Trust, Liverpool. Exclusion criteria included RA patients and infection. Formalin fixed paraffin embedded synovial tissues were sectioned at 5μm and stained with haematoxylin and eosin. Images of all tissues were captured and anonymised and used to assess synovitis in all tissues according to the criteria of Krenn et al (2006). Histological microimages of each complete synovial tissue were captured at 20x and digitalised using an Aperio CS2 Digital Pathology Scanner. These images can be utilised for further comparative characteristics of synovial tissue histology in the OA, arthroscopy and trauma groups to underpins the involvement of synovial tissues in OA pathogenesis.