Data Set on efficacy of vagina electrical resistance measurement and blood parameters in West African dwarf goats synchronized using Ovsynch and Double Prostaglandin protocol.
Description
One of the major constraints to good reproductive performance in West African dwarf (WAD) goats’ reproduction is low estrus detection. Ovsynch and Double prostaglandin are two common estrous synchronization protocols with main challenge of precise insemination timing. These data sets are results of study designed to measure the vaginal mucus electrical resistance (ERVM) as an indicator for estrus, ovulation, and insemination in 12 WAD does. Animals were randomly divided into; Group 1 (G1) comprised 6 does which were treated with two doses of prostaglandin analog (PGF2α) administered 11 days apart while Group 2 (G2) comprised 6 does that were synchronized using the Cosynch protocol (Day 1 – GnRH, Day 7 - PGF2α and Day 9 – GnRH). The VER reading was determined using Draminski heat detector. Per animal, the doe was restrained, using the index finger and thumb, the vulva was opened and the heat detector (disinfected using Savlon and dried with paper towel) was inserted at angle 45odegree into the vagina until it meets the fornix part of the vagina. The probe was then rotated twice and then the VER reading taken. The VER readings of the animals were taken 12 hourlies (Morning 6 – 7 AM and evening 6 – 7 PM) throughout the period of the experiment. Blood samples were collected from WAD doe in Group 1 on day 0, 7, 11, 12, 13 and 14 while those in Group 2 on day 0, 7, 9, 10, and 11 respectively. Mean vaginal electrical resistance (VER) values ranged from 405 ± 39 to 788 ± 160 in Group 1 and 352 ± 25 to 1098 ± 166 in Group 2. Estrus onset correlated with lowest VER post-treatment, with 40% in both groups showing signs within 36 hours, and patterns persisted. Progesterone declined and spiked in Group 1 on days 7-11, then dropped sharply on days 12-13; estradiol rose as progesterone fell. Group 2's progesterone spiked on day 10, inversely related to estradiol. A consistent pattern emerged: low VER, high estradiol, and low progesterone signified estrus. Day 13 saw 40% of Group 1 does in estrus, reflecting low VER and high estradiol (190.6 ± 31.4 µmol/l), similarly in Group 2. The current data contributes to available reports on efficacy of VER, estradiol, and progesterone as reliable predictors of estrus onset and highlights their role in reproductive management. Data can be used for further studies, validation of similar reports in ruminant reproduction and application of timed artificial insemination protocols.
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Steps to reproduce
Experimental Design Following the acclimatization, the WAD does were ear tagged and randomly assigned to the two groups by balloting. Group 1 (G1) comprised 6 does which were treated with two doses of prostaglandin analog (PGF2α) administered 11 days apart while Group 2 (G2) comprised 6 does that were synchronized using the Cosynch protocol (Day 1 – GnRH, Day 7 - PGF2α and Day 9 – GnRH). Data Collection Determination of VER reading The VER reading was determined using Draminski heat detector. Per animal, the doe was restrained, using the index finger and thumb, the vulva was opened and the heat detector (disinfected using Savlon and dried with paper towel) was inserted at angle 45odegree into the vagina until it meets the fornix part of the vagina. The probe was then rotated twice and then the VER reading taken. The VER readings of the animals were taken 12 hourlies (Morning 6 – 7 AM and evening 6 – 7 PM) throughout the period of the experiment. Blood collection Blood samples were collected from WAD doe in Group 1 on day 0, 7, 11, 12, 13 and 14 while those in Group 2 on day 0, 7, 9, 10, and 11 respectively. The WAD doe was restrained, and the neck area was swabbed with cotton wool impregnated with methylated spirit. Using digital pressure, the jugular vein was identified and 5mls of blood was collected using a sterile syringe and needle. The blood was immediately dispensed into a vacutainer tube without any anticoagulant, after which it was slanted at angle 30ᵒC to enable the gathering of the serum before centrifuging. The serum was collected after centrifuging at 1,500 rpm for 15 min and stored at −80°C. The serum sample was equilibrated at room temperature for half an hour before the standard procedure for detection of blood concentration of progesterone and estradiol using an enzyme-linked immunosorbent assay (ELISA) was followed. The Progesterone and Estradiol ELISA kits (NovaTec Immundiagnostica GmbH, Germany) were used to determine the concentrations of progesterone and Estradiol in each of the blood samples.