Thermal stress impairs photophysiology and redox balance in the kelp Lessonia spicata - Results Navarrete et al. Scientific Reports
Description
This dataset contains the raw data supporting the results presented in the article “Thermal stress impairs photophysiology and redox balance in the kelp Lessonia spicata”. The data correspond to physiological, biochemical, and molecular responses of the kelp Lessonia spicata exposed to thermal stress under controlled experimental conditions. The dataset is organized in an Excel file with three worksheets: 1. Physiological responses: Includes photophysiological parameters derived from photosynthesis–irradiance curves and chlorophyll fluorescence measurements. 2. Biochemical responses: Contains measurements related to pigment content and oxidative stress indicators. 3. Molecular responses: Includes relative expression levels of genes associated with photosynthesis, stress response, and metabolic pathways. Each row represents an individual replicate measurement. The variables Season time and Hours indicate the experimental sampling period and sampling time during the experiment. These data were generated to evaluate the impact of thermal stress on photophysiological performance, oxidative balance, and gene expression in Lessonia spicata, contributing to understanding the physiological mechanisms underlying thermal tolerance in kelp species.
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Sample collection Sporophytes of Lessonia spicata were collected in September 2023 from the rocky intertidal zone at Cochoa Beach (32°57′19″ S; 71°32′52″ W), located north of Valparaíso Bay, Chile. Approximately 50 individuals (20–50 cm length) were sampled. To avoid pseudoreplication, each replicate corresponded to tissue taken from different individuals separated by at least 1 m. Transport and acclimation Samples were transported under cooled conditions (13–15 °C) to the Laboratory of Environmental Research (LACER) at Universidad de Playa Ancha. Algae were maintained with aeration and seawater exchange and acclimated for 48 h at 15 °C under a 12:12 h light:dark photoperiod simulating natural radiation conditions (PAR, UVA and UVB). Experimental design and temperature treatments Blade segments from independent individuals were placed in experimental tanks. Two temperature treatments were applied: Control: 15 °C Warming treatment: 18 °C (+3 °C scenario consistent with projected ocean warming). Tanks (n = 3 per treatment) were randomly assigned, and temperature was controlled using a chiller system. Seawater was replaced every three days and fronds were repositioned to avoid self-shading. Sampling schedule Measurements were performed at three experimental times: 24 hours (day 1) 7 days 14 days Sampling was conducted at 10:00 local time. Independent fronds were used at each sampling time to avoid repeated measurements on the same tissue. Tissue for biochemical and molecular analyses was immediately frozen in liquid nitrogen. Photophysiological measurements Photosynthetic performance was evaluated using chlorophyll fluorescence with a pulse-amplitude-modulated fluorometer (Junior-PAM). Parameters obtained included: Maximum quantum yield (Fv/Fm) after dark adaptation Maximum electron transport rate (ETRmax) from rapid light curves Photosynthetic efficiency (αETR) Maximum non-photochemical quenching (NPQmax). Biochemical analyses Biochemical responses were quantified using spectrophotometric assays, including: Chlorophyll a and chlorophyll c concentrations Total phenolic compounds (Folin–Ciocalteu method) Antioxidant capacity (DPPH assay) Reactive oxygen species (ROS) Lipid peroxidation measured as malondialdehyde (MDA) using the TBARS method. Data organization Raw measurements were compiled into an Excel dataset containing physiological, biochemical, and molecular response variables. Each row represents an independent replicate corresponding to a different individual thallus and sampling time. Statistical analyses Experimental data were analyzed using two-way ANOVA with temperature and exposure time as factors. Additional multivariate analyses (PCO) were used to evaluate integrated physiological and biochemical responses to thermal stress.
Institutions
- Universidad de Playa Ancha de Ciencias de la EducaciónValparaíso, Valparaíso
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Funders
- FONDECYT 11180197, ANID, Chile.Grant ID: 11180197