Biosolid data of Dry Sanitation System from Berriozabal, Chiapas, Mexico

Published: 5 June 2024| Version 1 | DOI: 10.17632/d4yhphvf8w.1


The dry sanitation system (DSS) is an alternative eco-technique for water care in marginalized communities where water resources are limited. Additionally, the biosolids generated can be used for soil restoration and agricultural purposes. In order to reuse these biosolids, the presence and quantity of fecal coliform bacteria must be determined. The amount of coliforms found in a sample can be used to assess unhealthiness of biosolids from DSS. Within fecal coliforms, E. coli is classified into different pathotypes according to its pathogenicity mechanism such as ETEC, EPEC, EIEC, STEC, EAEC and DAEC. In this study, biosolids from DSS at Berriozabal, Chiapas, Mexico, were analyzed for a year. Fecal coliforms were measured with the most probable number technique, while E. coli pathotypes were investigated by PCR. Fecal coliforms were isolated from 50% of the biosolids simples and two strains of E. coli with the bfpA virulence gene of the enteropathogenic E. coli (EPEC) pathotype were isolated. Most of the biosolids obtained from DSS can be used as compost for ornamental plants. These analyzes are important to determine the presence of coliforms in biosolids and propose strategies that reduce this type of microorganisms in biosolids generated with this eco-technique, so that they can be used for agricultural purposes in these marginalized communities of Chiapas.



Universidad de Ciencias y Artes de Chiapas


Escherichia coli, Environmental Microbiology, Mexico, Ecological Sanitation


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