Processed Lipidomics data: Lung adenocarcinoma vs. normal lung tissue (human)
Description
For lipidomic analyses, 22 normal lung tissues and 29 tumor tissues from adenocarcinoma patients undergoing lung resection were analyzed. Samples were processed as detailed in Hoppstädter et al. (2021, DOI: https://doi.org/10.1016/j.ebiom.2021.103578). The analysis of lipids was performed by direct flow injection analysis (FIA) using a triple quadrupole mass spectrometer (FIA-MS/MS; QQQ triple quadrupole; Micromass Quattro Ultima, Waters) and a hybrid quadrupole-Orbitrap mass spectrometer (FIA-FTMS; high mass resolution; Qexactive Orbitrap, ThermoFisher Scientific). FIA-MS/MS (QQQ) was performed in positive ion mode using the analytical setup and strategy described previously (Liebisch et al., 2006, doi:10.1016/j.bbalip.2005.12.007; Liebisch et al., 2004, doi: 10.1016/j.bbalip.2004.09.003 ). The Fourier Transform Mass Spectrometry (FIA-FTMS) setup and data processing details are described in (Höring et al., 2019, doi:10.1021/acs.analchem.8b05013). For PE, PE P, PS, PI, PG, LPC, LPE, SM, Cer, and HexCer, QQQ data were analyzed. For PC, PC O, DG, TG, FC, and CE, FTMS data were used.
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Steps to reproduce
See Hoppstädter et al., 2021 (DOI: 10.1016/j.ebiom.2021.103578) for details.