High-speed single molecule imaging of membrane proteins in rat basophilic leukemia cells.

Published: 2 March 2020| Version 2 | DOI: 10.17632/d5z3gt7xs9.2
Keith Lidke


A high-speed fluorescence microscope operating at a 490 Hz frame rate was used to image two different membrane proteins- the high-affinity IgE receptor FcεRI, a transmembrane protein, and an outer-leaflet GPI-anchored protein. The IgE receptor was imaged via IgE labeled with Janelia Fluor 646 and the GPI-anchored protein was imaged using a GPI-GFP fusion protein and an ATTO647N labeled anti-GFP nanobody. Data was collected for both proteins in untreated cells and cells that had actin stabilized by phalloidin.