Deferoxamine deconditioning increases neuronal vulnerability to hemoglobin

Published: 26 February 2020| Version 1 | DOI: 10.17632/d9pwn2sx2v.1
Raymond Regan,
Denggao Peng,
Cindy Acon Chen,
Deepa Ruhela,
Yang Li


Hypothesis: Deferoxamine pretreatment and abrupt withdrawal increases the vulnerability of neurons to hemoglobin. Model: Primary cortical cell cultures (murine); cultures were pretreated with deferoxamine alone, deferoxamine plus hemoglobin, or vehicle alone for 24 hours. After wash, cultures were then treated with hemoglobin alone, hemoglobin plus antioxidants, or hemoglobin plus deferoxamine or apoferritin. Outcome measures: Lactate dehydrogenase (LDH) release, culture nonheme iron, cell expression of ferritin, heme oxygenase-1 and glutathione peroxidase-4.