Deferoxamine deconditioning increases neuronal vulnerability to hemoglobin
Published: 26 February 2020| Version 1 | DOI: 10.17632/d9pwn2sx2v.1
Contributors:
Raymond Regan, Denggao Peng, Cindy Acon Chen, Deepa Ruhela, Yang LiDescription
Hypothesis: Deferoxamine pretreatment and abrupt withdrawal increases the vulnerability of neurons to hemoglobin. Model: Primary cortical cell cultures (murine); cultures were pretreated with deferoxamine alone, deferoxamine plus hemoglobin, or vehicle alone for 24 hours. After wash, cultures were then treated with hemoglobin alone, hemoglobin plus antioxidants, or hemoglobin plus deferoxamine or apoferritin. Outcome measures: Lactate dehydrogenase (LDH) release, culture nonheme iron, cell expression of ferritin, heme oxygenase-1 and glutathione peroxidase-4.
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Institutions
University of Maryland School of Medicine
Categories
Neurotoxicity, Stroke, Subarachnoid Hemorrhage, Iron, Intracerebral Hemorrhage