Metabolites differentiating controls from PCOS participants
Supplementary Table S1. VIP list obtained from OPLS-DA analysis of PCOS and controls Supplementary table S2. Metabolites differentiating controls from PCOS participants
Steps to reproduce
Tandem mass spectrometry was performed for targeted metabolomics of plasma samples using Biocrates MxP® Quant 500 Kit (Biocrates, Innsbruck, Austria) at the Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM). FIA-MS/MS was utilized to measure lipids using a 5500 QTRAP® instrument with an ESI source (AB Sciex, Darmstadt, Germany). LC-MS/MS utilizing the same 5500 QTRAP® instrument was used for measuring small molecules as described previously . Appropriate MS software (Sciex Analyst®) was used to quantify data that was then imported into Biocrates MetIDQ™ software for calculation of analyte concentrations, assessment and compilation of data.