Knockdown of EXT1 perturbs ER morphology

Published: 28 January 2021| Version 1 | DOI: 10.17632/ddwry7xn34.1
Jean-Claude Twizere


ATL1, RTN4, Lpn1, Sec23a, Sec16b markers localization in Cos7 cells knockdown for EXT1 compared to control cells


Steps to reproduce

-Generate EXT1 and control knockdown Cos7 cells using shRNA as described in the referenced article -Transfect the cells with indicated ER markers -24H post-transfection, fix cells with 4% paraformaldehyde in PBS for 15 min at RT -Image the cells with a Leica TCS SP8 microscope using the 100x oil objective. Images were taken at 2068 x 2068 pixel resolution and deconvoluted with Huygens Professional software.