Inflammatory potential of gut microbiota from monozygotic twin pair discordant for ulcerative colitis

Published: 11 February 2022| Version 1 | DOI: 10.17632/dfg449h2wt.1
Contributor:
Jesper Havelund

Description

Gut microbiota perturbations are common in inflammatory bowel disease (IBD), including ulcerative colitis (UC), but it is unclear whether this is a result of the disease or a contributing factor. In twin pairs discordant for IBD, the healthy co-twin is at an increased risk of IBD and has a gut microbiota more similar to IBD patients than healthy individuals. Moreover, appropriate medical treatment may reduce the perturbations. We, therefore, transferred stool from a discordant twin pair, i.e. one healthy and one treated for UC, to germ-free mice by the inoculation of pregnant dams. Dextran sodium sulfate was used to induce colitis in the pups. Offspring of mice inoculated with stool from the UC twin had a lower disease activity index (DAI) and gut inflammation and microbiota with higher alpha diversity and a more anti-inflammatory profile, with the exclusive presence of the anti-inflammatory Akkermansia species, compared to offspring of mice inoculated with stool from the healthy co-twin. These results indicate that the healthy twin had a more inflammatory microbiota than the twin treated for UC. Our study suggests that individuals susceptible to IBD have a more inflammatory gut microbiota. Future studies should assess the potential use of individual gut microbiota inflammatory profiles in personalised medicine.

Files

Categories

Gut Microbiome

Licence