Bastos data OPNc and BBB-on-a chip
Description
This raw data contains de data generated that comprehends the research article we are submmiting. We developed a multicellular human blood–brain barrier-on-a-chip model toinvestigate the contribution of OPNc to leukemic cell transmigration. The microfluidic system recapitulates key structural and functional features of the blood–brain barrier (BBB), including endothelial, pericyte, and astrocyte interactions, and exhibits selective barrier properties. Using this system, we demonstrated that RS4;11 leukemic cells adhere to, infiltrate, and transmigrate across the BBB. Silencing of OPNc significantly reduced leukemic cell migration and invasion in transwell assays and was associated with altered cellular morphology, suggesting impaired migratory plasticity. OPNc-silenced cells showed consistent trends toward reduced infiltration and increased circularity rates. Altogether, these findings support a a key role for OPNc in regulating leukemic cell behavior associated with CNS infiltration and establishing the BBB-on-a-chip as a physiologically relevant platform for studying leukemic dissemination.
Files
Steps to reproduce
data contain real time PCR assays regarding OPNc levels in B-ALL cell line; also contain quantification od migration and invasion of this leukemia cell line depending on OPN-c levels; BBB-on- a chip has been tested for its barrier selectivity and cell migartion potential through the BBB layer in response to OPN-c silencing.