Peripheral inflammation induces allodynia and increases c-Fos expression in the basolateral amygdala
Published: 13 September 2018| Version 1 | DOI: 10.17632/djvyxd7syt.1
Contributors:
julio cesar morales medina, , , Description
The research hypothesis is that peripheral inflammation will cause an increase in c-Fos expression in brain areas involved in the control of pain. We induced mechanical allodynia with the administration of the complete Freund’s adjuvant (CFA) in the hindpaw that resulted in mechanical allodynia at the third day post administration. A behavioral test was carried out and mechanical allodynia was observed. Two hours later, the rats were sacrificed and the brains collected for inmunohistochemical analysis.
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Steps to reproduce
Behavioral analysis using the von Frey tests and immunohistochemistry for c-Fos expression. All methods are described in detail in the manuscript.
Institutions
Kent State University, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional
Categories
Behavioral Neuroscience, Cellular Neuroscience, Animal Research