Bulk RNA sequencing data of macrophages treated with or without sFRP1

Published: 10 April 2024| Version 1 | DOI: 10.17632/dkgcwz3tnb.1
Contributor:
Xiangyun Yao

Description

Total RNA of BMDMs was isolated using the Trizol reagent. RNA extracted from non-treated BMDMs was marked as control while RNA from sFRP1-treated BMDMs was marked as experiment group. Then, transcriptome sequencing and analysis were performed at Oebiotech Co. Ltd (Shanghai, China). Differential expression analysis was performed using DESeq2 (1.24.0) analysis. P value<0.05 and fold change>2 were set as the threshold for significantly differential expression. All the differentially expressed genes were named as "all DEGs" in the Mendeley repository and the significantly differential genes were named as "Significant DEGs".

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Institutions

Shanghai 6th Peoples Hospital Affiliated to Shanghai Jiaotong University School of Medicine

Categories

RNA Sequencing

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