Data from: Species boundaries to the limit: validating species delimitation methods is critical to avoid taxonomic inflation in the genomic era
Description
This is a data repository containing the following files: Raw demultiplexed reads for all specimens used in the study: Species boundaries to the limit: validating species delimitation methods is critical to avoid taxonomic inflation in the genomic era
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Steps to reproduce
Genomic libraries were produced following Peterson’s et al. (2012) protocol for double-digest restriction site-associated DNA (ddRADseq) for 52 specimens. In short, we double-digested 500 ng of genomic DNA, using a pair of rare and common restriction enzymes (Sbf1 and Msp1, respectively). The resulting fragments were ligated with barcoded Illumina adapters and afterwards were pooled together in batches of eight samples. Fragments were then size-selected for a range between 415 – 515 bp and sequenced on an Illumina NextSeq 500, for 75 bp single-end reads. Raw Illumina reads were demultiplexed using Ipyrad v.0.9.62 (Eaton & Overcast, 2020)
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Funding
MCIN/AEI/10.13039/501100011033 and by the European Union NextGenerationEU/PRTR
FJC2021-046832-I
(MCIU/AEI/FEDER, UE)
PGC2018-098290-B-I00
MCIN/AEI/10.13039/501100011033 and by ERDF, a way of making Europe
PID2021-128901NB-I00
Ministerio de Ciencia, Innovación y Universidades, Spain
FPU18/04742
Ministerio de Ciencia, Innovación y Universidades
PRE2019-088729
“la Caixa” doctoral fellowship programme
LCF/BQ/DR20/11790007