Plasma Essential Amino Acid Levels in First Episode Psychosis at Baseline and After Antipsychotic Treatment

Description

The plasma levels of essential amino acids (EAA) such as phenylalanine (Phe), Trp, valine (Val), threonine (Thr), methionine (Met), leucine (Leu), isoleucine (Ile), lysine (Lys), and histidine (His) were measured in drug-naïve first episode psychosis (FEP) patients at diagnosis and after 10 weeks of antipsychotic treatment. Forty FEP patients were enrolled at baseline, with blood samples collected before and after a 10-week antipsychotic treatment period. Plasma EAA levels were measured using an LC/MS/MS method.

Steps to reproduce

Drug-naive first episode psychosis patients were recruited. The blood samples were obtained before initiating the treatment and after 10 weeks antipsychotic treatment. Lithium heparin containing tubes were used and blood samples were incubated at +4°C for 30 minutes and then centrifuged at +4°C for 5 minutes at 5000 rpm to obtain plasma preserved at -80°C until the analysis time. Those procedures were repeated before and after 10 weeks of antipsychotic treatment. The EAAs, Phe, Trp, Val, Thr, Met, Leu, Ile, Lys, His, and conditionally essential amino acid Tyr were measured by using a modified liquid chromatography/mass spectrometric (LC-MS/MS) method. More detalis can be found in our poublication with the same title.

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