Cluster-specific effects of non-medicinal herbal components on maternal fetal transmission by modified tryptophan metabolism

Published: 22 April 2024| Version 1 | DOI: 10.17632/dr5yjtshs9.1
Zhiyuan He


Metabolomics preprocessed data, the data set is related to figure 6, Extended Data figure S4-6.


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Feces were thawed on an ice bath to reduce degradation. Approximately 5 mg of each lyophilized sample was weighed and transferred to a new 1.5-mL tube. Twenty microliters of a sample was extracted with 120 μL of cold methanol/acetonitrile (1:1, v/v) and vortexed for 1 min. The mixture was homogenized for 5 min and subsequently centrifuged at 12,000×g for 10 min at 4 °C. Adding 150 μL of 2-chloro-l-phenylalanine (4 ppm) solution prepared with 80% methanol water to redissolve the sample, filter the supernatant by 0.22 μm membrane and transfer into the detection bottle for further analysis. In addition, pooled QC samples were also prepared by combining 10 μL of each extraction mixture. Metabolite profiling and data processing in feces and serum samples were performed on a Vanquish UHPLC system (Thermo Fisher Scientific, USA). Chromatography was carried out with an ACQUITY UPLC-HSS T3 (2.1×100 mm, 1.8 μm) (Waters Corp., Milford, MA, USA). The column maintained at 40℃. Each sample was analyzed via UPLC-MS/MS in both positive and negative ionization modes to acquire the metabolite profiles. Mass spectrometric detection of metabolites was performed on Orbitrap Exploris 120 (Thermo Fisher Scientific, USA) with ESI ion source.


Beijing University of Agriculture


Traditional Chinese Medicine, Alternative Assessment, Veterinary Immunology