Volatile organic compounds emitted by the mycoparasitic fungi Hypomyces perniciosus and Cladobotryum mycophilum suppress the growth of white button mushroom (Agaricus bisporus)
Description
Hypomyces perniciosus and Cladobotryum mycophilum are mycoparasitic fungi that infect Agaricus bisporus (button mushroom) and cause wet bubble and cobweb diseases, respectively. In this study, the role of volatile organic compounds (VOCs) emitted by these fungal mycoparasites in the suppression of A. bisporus was investigated. Further, the VOCs of H. perniciosus and C. mycophilum were collected by headspace solid-phase microextraction (HS-SPME) procedure, and their components were analyzed by gas chromatography-mass spectrometry (GC-MS). The effect of ethanol vapor, a major volatile component in these mycoparasites, on the growth of A. bisporus was determined.
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The antifungal effect of VOCs emitted by the mycoparasites against A. bisporus was tested by using a dual-culture method. The volatile compounds released by the mycoparasites were collected separately by using headspace-solid-phase micro extraction technique and analyzed by GC-MS.