Mechanistic Insights into the Cis- and Trans-acting DNase Activities of Cas12a

Published: 04-12-2018| Version 1 | DOI: 10.17632/dvvxbf5v2p.1
Contributors:
Daan Swarts,
Martin Jinek

Description

Raw imaging & fluorescence data belonging to the manuscript 'Structural Mechanistic Insights into Cis- and Trans-acting deoxyribonuclease activities of Cas12a' by Swarts. Both raw image files and unprocessed .tif image files are uploaded. The .pzfx files are GraphPad Prism files used to generate graphs based on gel scans and band signal intensity with the same name. Please not that trans-activity assays do not also contain images of gels as generated fluorescence was measured directly as described in the STAR methods. For (raw) gel images used for Figure 5, please see the identical gels used for Figure S7 and S8. The excel file contains the raw Size Exclusion Chromatography absorption and fluorescence readouts. The .PDB and .mtz files corresponding to the manuscript are uploaded at the PDB database.

Files

Steps to reproduce

See STAR methods in published manuscript.