Data for : Transplacental Transfer of Organochlorine Pesticides: Concentration Ratio and Chiral Properties

Published: 24 July 2019| Version 1 | DOI: 10.17632/dx6ffmm8zn.1
Contributors:
Shanshan Yin,
Jianyun Zhang,
Fangjie Guo,
Lu Zhao,
Giulia Poma,
Adrian Covaci,
Weiping Liu

Description

The raw data reported here was used in the DIB dataset. Matched maternal serum, cord serum and placenta samples from 79 volunteer mothers and their infants were collected between November 2015 and March 2016 in Wuhan, China. Informed consent was given by all participants to collect the physical and sociodemographic data. For Pretreatment of the samples, An aliquot of sample (2 mL for serum samples, ~5 g for lyophilized placenta) was spiked with TCMX as internal standards (50 ng, IS). 0.5 mL of formic acid, 2.5 mL of ethanol and 10 mL of n-hexane/DCM (1:1, v/v) was then added in for the extraction. The mixture was thoroughly mixed, ultrasonic extracted for ten minutes, followed by centrifugation separation at 2000 rpm for ten minutes. The organic phase was then collected; the ultrasonic extraction procedure was repeated three times. The extract was evaporated under vacuum to ~1 mL, then cleaned up by column chromatography with pre-activated silica gel and Na2SO4. Targeted compounds were collected using another portion of 70 mL of n-hexane/DCM (1:1, v/v) elution. The elution was evaporated to near dryness in a GC sample bottle, then reconstituted in 50 μL of n-nonane. Total lipids (TL) in the serum was calculated: TL (g/l) = 1.12 × CHOL + 1.33 × TG + 1.48. The lipid weight of the placenta was determined gravimetrically. Organochlorine pesticides (OCPs) concentrations were then measured in the samples using GC-MS/MS. Enantiomeric fractions were also determined for chiral OCPs in the samples using GC-NCI-MS. Information about the recovery, MDL and MQL for the analytes in the serum and placenta samples were provided in Data File 1. For this spreadsheet, each line is a recovery calculated using standard spike blank matrix samples before/after extraction. For each type of blank matrix, at least 8 samples were run. The three spiking concentration were set at 20 ng/mL, 5 ng/mL and 0.5ng/mL. For TCMX, working as the internal standard in our study, only 20 ng/mL was selected. Results of the wet-weight basis concentration of OCPs in samples were provided in Data file 2. For this spreadsheet, each line is one mother-infant pair, and each column is the wet-weight basis concentration (in ng/mL) for placenta, cord serum and maternal serum samples. Personal information of the volunteers were replaced by a number in this spreadsheet to protect their privacy. Box-plots with dots representing the lipid adjusted concentration ratio of DDX metabolites (DDD+DDE) vs. the DDT parent compounds, and β-HCH vs. (α+γ)-HCH were provided in Data File 3. For this spreadsheet, each line is one mother-infant pair, and each column is the lipid-weight concentration ratio of β-HCH/(α+γ)-HCH and (DDD+DDE)/DDT for placenta, cord serum and maternal serum samples. Personal information of the volunteers were replaced by a number in this spreadsheet to protect their privacy.

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