IMMOBILIZATION OF PORCINE PANCREATIC LIPASE ONTO MAGNETIC NANOPARTICLES FOR ENZYMATIC TRANSESTERIFICATION OF OILS
Description
Enzymes are highly specific biocatalysts and their properties and application can be improved by immobilization methods. This type of technology reduces the cost of process, in addition to reusing the same enzymes for many cycles. Among the available enzymes, lipases stand out due to the ability to hydrolyze triglycerides in glycerol and fatty acids. The products of this reaction are used in various segments of industry, such as dairy products, detergents, bakery, pharmaceutical and biofuels. Magnetic nanoparticles were used as a support to immobilize the lipase and allow its recovery by applying an external magnetic field. This carrier enables use of magnetic separation for recovering of enzymes, reducing costs and allowing its reuse in continuous systems. In this context, the goal of this work was to immobilize porcine pancreatic lipase (PPL) onto magnetic carrier (Fe3O4-APTS). The Fe3O4-PPL immobilization yield was 78.94%. The physical and chemical properties of nanoparticles and immobilized lipase were characterized by TGA, XRD, SEM, IR, TEM and DLS. In addition, immobilized lipase exhibited significantly improved stability over a wide pH and temperature range compared to free lipase. The immobilized derivate also presented a good reusability, keeping 61.37% of its initial activity after 6 cycles of the reaction.