Productions of IL-4 and IL-10 were enforced via the function of IL-2 from anti-CD3 antibody-stimulated mouse spleen cells treated with caffeic acid phenethyl ester
Description
Figure 1. IL-2 productions from anti-CD3 antibody-stimulated spleen cells treated with CAPE or from untreated control cells. Figure 2. IFN-γ mRNA expression and the productions from anti-CD3 antibody-stimulated spleen cells treated with CAPE. Figure 3. IL-4 mRNA expression and the productions from anti-CD3 antibody-stimulated spleen cells treated with CAPE. MRNA expression (A) and the protein productions (B) of IL-4 from untreated control cells are described. Figure 4. IL-10 mRNA expression and the productions from anti-CD3 antibody-stimulated spleen cells treated with CAPE. MRNA expression (A) and the protein productions (B) of IL-10 from untreated control cells are described in blue bars; and those from the cells treated with 11μM of CAPE are described in orange bars, respectively. Figure 5. Effects of IL-2 upon the expression and production of IL-4 in the stimulated spleen cells treated with CAPE. (A) MRNA expression of IL-4 from the stimulated spleen cells in the different culture conditions. Ordinate, Relative expression level of IL-4. (B) The production of IL-4 from the stimulated spleen cells in the different culture conditions for 96h. Figure 6. Effects of IL-2 upon the expression and production of IL-10 in the stimulated spleen cells treated with CAPE. (A) MRNA expression of IL-10 from the stimulated spleen cells in the different culture conditions. Ordinate, Relative expression level of IL-10. (B) The production of IL-10 from the stimulated spleen cells in the different culture conditions for 96h. Figure 7. Effects of CAPE upon the subsets of the stimulated spleen cells The ratio (%) of CD4+ cells (A), DC8+ cells (B), and CD4-CD8- cells (C) among lympho-gated populations of the stimulated spleen cells after 96h in the different culture conditions.
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Mouse spleen cells were stimulated by anti-CD3 monoclonal antibody in the presence of CAPE. Productions of cytokines were examined by an enzyme-linked immunosorbent assay (ELISA). The mRNA expression was examined by a reverse transcription-quantitative polymerase chain reaction (RT-PCR). The function of IL‑2 was examined using neutralizing antibodies. Subsets of spleen cells were examined by flow cytometry.