Supporting data for “Beyond a uniform defense: Pattern-recognition receptor identity shapes immune outputs in sweet cherry”

Description

Plant pattern-triggered immunity (PTI) is generally considered a conserved defense mechanism activated after the recognition of microbe-associated molecular patterns (MAMPs). However, whether different pattern-recognition receptors (PRRs) simply trigger a common immune response or instead bias immunity toward distinct regulatory states remains poorly understood, particularly in woody perennial species. We hypothesized that activation of the bacterial receptor PaFLS2 by flg22 and the fungal receptor PaEIX2 by xyn11/EIX establishes partially distinct immune programs that differentially shape transcriptional regulation, phytohormone accumulation, and disease resistance in sweet cherry (Prunus avium). To test this hypothesis, we combined molecular, structural, biochemical, and physiological approaches. The dataset includes the cloned nucleotide and amino acid sequences of PaFLS2, sequence alignments and domain analyses, orthology-informed transcription factor–target regulatory networks projected from experimentally validated Arabidopsis thaliana interactions, gene expression analyses by RT-qPCR, phytohormone and metabolite measurements, pathogen susceptibility assays, and the integrated datasets used for principal component analysis (PCA) and K-means clustering. The data demonstrate that flg22 and xyn11/EIX do not simply activate a uniform PTI response. Instead, the two elicitors induce partially overlapping but distinct transcriptional, hormonal, and physiological responses that are associated with different pathogen resistance outcomes. Multivariate analyses further reveal that these responses segregate into distinct molecular states, supporting the conclusion that PRR identity contributes to immune specificity in sweet cherry. The deposited files include both processed datasets and supporting information required to reproduce the analyses presented in the associated manuscript. They are intended to facilitate transparency, reproducibility, and reuse in studies of plant immunity, receptor biology, transcriptional regulation, phytohormone signaling, systems biology, and defense responses in perennial crops. Researchers may use these data for comparative analyses, orthology-based regulatory inference, meta-analyses, or as reference datasets for studies of PRR-mediated immunity in Prunus and other plant species.

Institutions

• University of O'Higgins

  O'Higgins Region, Rancagua

Categories

Funders

• Agencia Nacional de Investigación y Desarrollo

  Santiago

  Grant ID: ANILLO ACT190001, ANILLO ACT192073 and ACT210004, FONDECYT 11200934, 11260557, 1230194 and 1251814, PAI 7190027, IDI23I10052, Postdoctoral grant ANID 3220080, ICN2021_044, ACE210010 and FB210005.

Licence