Gut microbes contribute to variation in foraging intensity in the honey bee, Apis mellifera.
Description
Associated with manuscript by Vernier et al, entitled: Gut microbes contribute to variation in foraging intensity in the honey bee, Apis mellifera. Preprint DOI: https://doi.org/10.1101/2023.08.31.555606 Hypothesis: Gut microbes modulate honey bee division of labor. See Vernier et al for more information. Notable findings: Nurses and foragers consistently differ in gut microbial community within experimental colonies, and single microbe inoculations with Bifidobacterium asteroides, Bombilactobacillus mellis, and Lactobacillus melliventris cause effects on group and individual level foraging intensity. See Vernier et al for more information. 16S rRNA sequencing data: 16S rRNA sequencing sample information for each sequencing run (December 2020, December 2021, July 2021), ASV and taxonomy tables for each 16S rRNA sequencing run. Behavioral data: Single microbe inoculation information (dates of inoculation, dates of behavioral monitoring, barcode information, colony location, etc.), Single microbe forager data (information on individual foragers, elite forager status, etc.), raw entrance data (all incoming and outgoing events for each barcode in each experimental colony with dates and times in Unix and human readable formats). Modular Pupation Plate: Design file for modular pupation plates.
Files
Steps to reproduce
See Vernier et al methods.