Determination of de novo RNA sequence of North Pacific krill
Published: 26 February 2020| Version 2 | DOI: 10.17632/f2xhbjhddj.2
Contributor:
Hidetoshi YamadaDescription
Total RNA was purified from E. pacifica using with an RNeasy Lipid tissue mini kit. The library of E. pacifica for next generation sequencing was made using with a TruSeq RNA library prep kit v2 (Illumina). RNA purification and library preparation were performed according to the manufacturers’ instructions. The library was analyzed by Miseq using a Miseq reagent kit v3 (600 cycle) (Illumina). The fastaq data was assembled by Trinity.
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DNA Sequencing