Determination of de novo RNA sequence of North Pacific krill

Published: 26 February 2020| Version 2 | DOI: 10.17632/f2xhbjhddj.2
Contributor:
Hidetoshi Yamada

Description

Total RNA was purified from E. pacifica using with an RNeasy Lipid tissue mini kit. The library of E. pacifica for next generation sequencing was made using with a TruSeq RNA library prep kit v2 (Illumina). RNA purification and library preparation were performed according to the manufacturers’ instructions. The library was analyzed by Miseq using a Miseq reagent kit v3 (600 cycle) (Illumina). The fastaq data was assembled by Trinity.

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DNA Sequencing

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