Data for green synthesis and characterization of silver nanoparticles from ethanolic extracts of leaves of Annona muricata
Description
This data was obtained from the synthesis and characterization of highly stable silver nanoparticles from ethanolic extracts of leaves of Annona muricata. AgNPs can be synthesized from Annona muricata leaves (AgNPs-L) using AgNO3 solution. The AgNPs can be characterized using spectroscopy and microscopy techniques. The data is composed of images, Microsoft excel data sheets, tables, among others. The data can be used by researchers undertaking similar research involving synthesis of AgNPs using leaves extracts of Annona muricata or any other plant species belonging to the same family. It can also be used to compare results from similar studies and or even replicate research as presented herein.
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Steps to reproduce
Fresh leaves of Annona muricata can be collected from the wild, washed with clean water, and then chopped into small pieces and allowed to air dry for a period of 2 weeks. The dried leaves can then be milled into a powder using an electric grater. The extraction can be achieved the plant tissue homogenization method in ethanol of about 70 – 100% v/v for about 48 hours. The dark green ethanolic extracts of Annona muricata leaves can then be filtered and kept at 4oC until use. 1mM AgNO3 solution can then be prepared in distilled from AgNO3 at a percentage purity of about 99.7%. An appropriate volume of the filtered leaves extract can be mixed with an appropriate volume of AgNO3 solution at a recommended concentration and mixed thoroughly, forming a uniform mixture. The mixture can then be rested at room temperature in the dark storage cabinets for up to about 72 hours, with continuous monitoring. After about 3hours, the mixture can then be observed to start changing from dark green to yellowish brown. After about 72 hours, the mixture will completely have changed colour to dark brown. This color change is visual evidence of formation of AgNPs or reduction of silver ions into AgNPs due to the excitation of surface plasmon vibration. The synthesis of AgNPs from the ethanolic extract of leaves of Annona muricata can be confirmed by ultraviolet - visible spectroscopy (UV/VIS) in the range of between 300nm to 650nm with ethanol as a blank. The UV/VIS spectra can be analyzed by plotting graphs of absorbance against wavelength. The appearance of absorption spectra between 400nm – 450nm confirms formation of AgNPs. pH stability can be studied by subjecting the AgNPs solutions to different pH conditions and measuring the absorbance spectra on UV/VIS. The pH can be adjusted by either adding drops of 1N NaOH or 1N HCl until the desired pH was achieved as observed on the pH meter. The UV/VIS spectra can be analyzed by plotting graphs of absorbance against wavelength. The appearance of absorption spectra between 400nm – 450nm confirms the pH stability of the AgNPs. The temperature stability can be studied by subjecting appropriate volumes of the AgNPs suspension in boiling tubes to different temperature conditions by heating in a digital water bath for about 3 minutes each and measuring the absorbance spectra on the UV/VIS in a scan range of 350nm to 650nm. Absorption maxima are recorded. For storage stability, the formed AgNPs suspension can be aliquoted in to different 5 mls of the AgNPs suspension. The suspensions in the tubes can then be stored at different temperatures for the desired period of time and then analyzed on UV/VIS. Functional group analysis can be conducted using FTIR spectroscopy, and data exported to Origin Software or a similar one for analysis. Spectra can be interpreted using the IR charts. Crystallite size determination can be done using XRD analysis and data analyzed using the Match! Software.