Data for: Encephalomyocarditis virus 2C protein antagonizes interferon-β signaling pathway through interaction with MDA5

Published: 23 November 2018| Version 1 | DOI: 10.17632/f5dypn7vh3.1
Contributors:
Juan Bai,
Zhongbao Song,
Hui Fan,
Xuewei Liu,
Liang Li,
Ping Jiang

Description

Sep 30, 2018 Dear Editors-in-Chief, Dr Gorisr Thank you so much for forwarding me the valuable reviewer’s comments. I have carefully addressed all of the issues raised by the referee as your comments. The manuscript was revised as follows (blue words): Reviewer 1 1. EMCV has four capsid proteins and eight non-structural proteins. In the study, only seven non-structural proteins were cloned into expression vectors and their inhibitions of IFN-β induction were studied. How were the other proteins? Reply: The comment is reasonable. It has been discussed in the fifth paragraph of DISSUSION 2. Luciferase assays results indicate 2C protein of EMCV affects inhibition of the type I IFN signaling pathway by MDA5 or RIG-I. However, the results of co-immunoprecipitation and confocal microscopy showed EMCV 2C only targets and interacts with MDA5. What is the possible mechanism of 2C protein inhibiting RIG-I-mediated IFN-β promoter activity? Reply: The comment is reasonable. It has been discussed at the end of the third paragraph of DISSUSION 3. Minor error, in page 9, line 368, the” (10-325)” should be deleted. Please check the full text carefully. Reply: The comment is reasonable. It has been correct. Reviewer 2 4. The introduction is an enumeration of facts, and not focused on the main studies of the paper. Sometimes is also misleading. For example, the sentence: “Nevertheless, whether EMCV regulates the innate immune response is not known.” Is incorrect, as there are described mechanisms how EMCV inhibits innate immune responses. Reply: The comment is correct. It has been addressed. 5. Most of the literature will now use the name MAVS, and not VISA. Reply: The comment is reasonable. It has been addressed. In addition, we have checked the full text carefully in grammar and spelling. The revised manuscript with modifications marked with red color is enclosed. We hope that it can be published as soon as possible. I am looking forward to hearing from you. Thanks! Sincerely, Juan Bai, PhD, Associated professor College of Veterinary Medicine Nanjing Agricultural University Nanjing 210095 P.R. China baijuan@njau.edu.cn

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