Hierarchical Cluster Analysis for biodiesel (B7 B10) and IUU fuels in Malaysia using OPUS

Description

This summary encapsulates the step-by-step process of conducting Hierarchical Cluster Analysis (HCA) using OPUS 8.5 software, covering data preparation, analysis execution, and result interpretation. Software Launch: Open OPUS 8.5 software. Accessing Cluster Analysis: Navigate to the "Evaluate" dropdown menu. Selecting Cluster Analysis: Choose "Cluster Analysis" from the dropdown menu. Loading Method or Default: Load preferred method or proceed with default settings. Navigating to Spectra Reference Tab: Access the "Spectra Reference" tab within the Cluster Analysis interface. Adding Spectra: Import spectral data files into the software. Selecting Spectra: Choose specific spectra files for analysis. Preprocessing (If necessary): Apply preprocessing techniques such as Vector Normalization. Defining Analysis Region: Specify spectral region range for analysis. Initiating Cluster Analysis: Click "Analysis Cluster" to start the analysis process. Reviewing Analysis Report: Access and view the generated cluster analysis report. Exploring Analysis Reports: Explore different report formats (Dendritic, Histogram, Basic Data) for insights. Data Visualization: Utilize visualization tools for further documentation and reference. This summary encapsulates the step-by-step process of conducting Hierarchical Cluster Analysis (HCA) using OPUS 8.5 software, covering data preparation, analysis execution, and result interpretation.

Steps to reproduce

1. Launch the OPUS 8.5 software on your computer system. 2. Access Cluster Analysis: Once the software is opened, navigate to the "Evaluate" dropdown menu. 3. Select Cluster Analysis: From the dropdown menu, select "Cluster Analysis" and click on it. 4. Load Method or Select Default: If a specific method for cluster analysis is available and preferred, load it. Otherwise, proceed with the default method, which is typically Standard (Euclidean Distance) with data processing set to Vector Normalization. 5. Navigate to Spectra Reference Tab: Click on the "Spectra Reference" tab within the Cluster Analysis interface. 6. Add Spectra for Analysis: Select the "Add Spectra" tab and browse your computer/directory to locate the folder containing the spectra you wish to analyze. Ensure that the folder is named appropriately and that the format of the spectra is compatible with the software (usually .0 format). 7. Select Spectra: Choose the spectra you want to include in the analysis and add them to the software. 8. Preprocessing Selection (If necessary): Determine the preprocessing mode suitable for your analysis, such as Vector Normalization. Optionally, adjust the range of the spectral region by utilizing interactive region selection tools or by manually entering the desired region range. 9. Define Analysis Region: Specify the spectral region range for analysis. 10. Initiate Cluster Analysis: Once the analysis region range is set, click on the "Analysis Cluster" button to start the analysis process. 11. Review Analysis Report: After the analysis is completed, click on the "Report" button to view the cluster analysis report. 12. Explore Analysis Reports: The cluster analysis report can be displayed in various formats, including Dendritic, Histogram, and Basic Data. Explore these different formats to gain insights into the clustering results. 13. Data visualization: For further reference and documentation, detailed analysis data can be printed directly from the software interface.

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