Regulatory dynamics determine cell fate following abrupt antibiotic exposure. Schultz et al

Published: 24 October 2017| Version 1 | DOI: 10.17632/fbwd37ynp8.1
Contributor:
daniel schultz

Description

Data_S1 is a Matlab file containing the growth curves of six liquid-culture experiments used in Figures 1 and 2, obtained with an automated robotic system at 30ºC. The 'Experiment' field includes a reference to the panels in the main text where the data is used, and the 'Info' field specifies how tetracycline was added to the cultures. The 'Data' field contains optical density (OD) and fluorescence (when applicable) measurements for each strain used in the experiment, together with vectors specifying the gradients of IPTG and tetracycline used (when applicable), the time of the measurements, indication of each type of measurement performed and times of IPTG induction (in Fig. 1F). A detailed description of the methods and strains used is given in the STAR Methods section. Data_S2 contains the image files obtained with the microfluidic device, where cells carrying the native tet resistance mechanism were exposed to a step increase of 70μg/ml tetracycline at time zero. Images were taken every 10 minutes at 10 different positions in 3 different fluorescence channels. CFP was expressed constitutively and used for image segmentation. GFP was expressed from the same promoter as TetR and mCherry was expressed from the same promoter as TetA. The file name indicates the time, position and fluorescence channel. A detailed description of the experiment and the strain used is given in the STAR Methods section.

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Bacterial Model, Imaging in Microfluidic Devices

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