Improved split TEV GPCR beta-arrestin recruitment assays

Published: 29 December 2022| Version 1 | DOI: 10.17632/fd45dm2bz5.1
Contributors:
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Michael Wehr

Description

Data set for "Improved split TEV GPCR beta-arrestin recruitment assays via systematic analysis of signal peptide and a beta-arrestin binding motif variants" as published in Wu et al., Biosensors 2023, 13(1), 48; https://doi.org/10.3390/bios13010048 Data contain firefly luciferase readings from split TEV GPCR beta-arrestin-2 recruitment assays in four cell lines (HEK-293, U-2 OS, HeLa, and PC12-tetOff cells). Data from dose response assays for GLP1R and GCGR were performed in HEK-293 cells (data files "DRC").

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Steps to reproduce

Plasmids encoding G protein-coupled receptors (GPCRs) linked to split TEV assay components were transiently transfected into HEK-293, U-2 OS, HeLa, and PC12-tetOff cells and stimulated with cognate ligands for 6h. Cells were lysed in Passive Lysis Buffer (Promega). Firefly luciferase activity was measured in a Mithras LB 940 Microplate Reader (Berthold Technologies) using the software MicroWin2000. See Methods section in Wu et al., Biosensors 2023, 13(1) for details.

Institutions

Ludwig-Maximilians-Universitat Munchen

Categories

Drug Discovery, G Protein-Coupled Receptor, Bioassay

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