Bioconversion of food waste to biocompatible wet-laid fungal films

Published: 23 August 2021| Version 1 | DOI: 10.17632/fdy8pgtv3b.1
Contributors:
, Tiffany Abtibol, Ana Oliveira, Mikael Magnusson, Karin Adolfsson, Sofie Svensson, Jorge Ferreira, Minna Hakkarainen,

Description

The fungus Rhizopus delemar grown on bread waste, in a submerged cultivation process, was wet-laid into films. The fungal cell wall was isolated by alkali or enzymatic treatments. The biomass was also subjected to a heat treatment to deactivate biological activity. Treated biomass was homogenized by an iterative ultrafine grinding process and finally cast into films by a wet-laid process. Ultrafine grinding improved the densification of films and they showed tensile strengths of up to 18.1 MPa, a Young’s modulus of 2.3 GPa and a strain at break of 1.4%. Alkali treatment resulted highest tensile strength, with SEM images showing a dense and highly organized structure. In contrast, enzymatic or heat treatments resulted in films with less organized structures. Biocompatibility of fungal films was confirmed by a cell viability assay and fluorescent staining.

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Institutions

  • RISE Research Institutes of Sweden AB
  • Kungliga Tekniska Hogskolan Institutionen for Fiber- och Polymerteknologi
  • Karolinska Institutet Department of Physiology and Pharmacology
  • Hogskolan i Boras Akademin for textil teknik och ekonomi

Categories

Films (Surface Science), Bioconversion, Filamentous Fungus, Zygomycetes, Biocompatibility, Food Waste

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