Whole genome sequence data of a chitinolytic actinomycete, Streptomyces californicus TBG-201

Published: 21 April 2023| Version 1 | DOI: 10.17632/fgtz42yfh7.1
Contributors:
,
Kumaradasan Sreelatha Deepthi ,
,
Anandhavally Satheesan Anugraha

Description

The whole genome sequence data of a chitinolytic actinomycete, Streptomyces californicus TBG-201, is presented here. The organism was isolated from the soil samples of the Vandanam sacred groves of Alleppey District in Kerala (9.4946o N, 76.3311o E). Whole genome sequencing was done using the Illumina HiSeq 2500 sequencing platform with a 2 x 150bp pair-end protocol. The sequenced read was assembled using Velvet v. 1.2.10. The length of the genome assembly was found to be 7.99 Mb. The genome has 72.60% GC content, 6683 protein-coding genes, 31 rRNAs, 66 tRNAs, and 116 pseudogenes. The presence of biosynthetic gene clusters was identified using antiSMASH. The carbohydrate-active enzyme coding genes were identified by CAZy analysis using the dbCAN meta server. Genome annotation using NCBI Prokaryotic Genome Annotation Pipeline provides insights into its chitinolytic activity. The whole-genome sequence was submitted to NCBI under the accession number JAJDST000000000.

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The genomic DNA of S. californicus TBG-201 was extracted using the CTAB method. The Illumina TruSeq Nano DNA Library Prep Kit was used to prepare the library. De novo sequencing of the genome was performed using the Illumina HiSeq 2500 sequencing platform with a 2 x 150bp pair-end protocol. Genome assembly was done using ABySS v. 2.0.1, MaSuRCA v. 2.3.2, and Velvet v. 1.2.10. The genomic sequence was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) by using the best-placed reference protein set; the GeneMarkS-2+ annotation method. Biosynthetic gene clusters were predicted using AntiSMASH. Carbohydrate active enzymes were identified by CAZy analysis using the dbCAN meta server.

Institutions

University of Kerala

Categories

Genome Sequencing

Funding

Department of Biotechnology, Ministry of Science and Technology, India

BT/PR12720/COE/34/21/2015

University of Kerala

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