Selection of suitable reference genes for Quantitative Real-time PCR in Mortierela alpine CBS 754.68

Published: 09-11-2020| Version 1 | DOI: 10.17632/fhcr8j4b6h.1
hamid rezaa samadloiy


The first effort has been made in this research to determine Mortierella alpine stable housekeeping genes under given conditions. statistic analysis indicated that when cell entered the death phase, actin was of the most stable expression for normalization of the expression levels, while in defined medium whereby cells stood in growth phase, 28s and dihydropteridine reductase were identified as the appropriate reference genes to normalize the gene expression data