Structure, characterization of chitosan derived from T.telescopium data set

Published: 07-04-2020| Version 2 | DOI: 10.17632/fj57tvsr6p.2
Jebarani Rajathy


FTIR: The chitosan derived from T. telescopium was characterized using a Fourier Transform Infra-Red Spectrophotometer (FTIR). The spectrum showed peak at 873.75 cm-1, which could be assigned C-N stretching. The absorption peak at 1454.33 cm-1is assigned to C-H bending of the side chain –CH2OH. 1654.92 cm-1is a characteristic of bending vibration of N-H while 3637.75 cm-1 is characteristic of free O-H groups. The band at 1103.28 cm−1 is attributed to the C O C stretching. The spectrum is relatively similar to that of commercial chitosan and all these absorption peaks are typical of chitosan molecule. SEM: The structure and morphology of the chitosan was studied by SEM analysis. The SEM micrograph shows that there is a difference in the surface morphology between the gastropod and the commercial chitosan. For T. telescopium chitosan the micrograph shows a smooth and homogenous surface with clustering of uneven particles. At high magnification, the particles were observed to be irregular in shape and dispersed as flakes. XRD: The XRD pattern of chitosan exhib¬ited broad diffraction peaks at 2θ = 18º and 35º which are typical finger prints of semi-crys¬talline chitosan. The gastropod chitosan was found to have few different peaks compared to commercial grade chitosan, which may be attributed to the presence of minerals in the prepared chitosan; but the major XRD peaks recorded are the characteristics of formation of chitosan compound.