Raw CT Value-QPCR and Cell Number of Heparin Dose Response in HER2-positive breast cancer

Published: 7 December 2022| Version 2 | DOI: 10.17632/fkfh69gnty.2
Contributor:
Hoang Son Pham

Description

This dataset characterises the changes in 2D breast cancer models of hallmark gene expression under basal growth conditions and cell proliferation in the added heparin cultures. For the cell proliferation changes, the two breast cancer cells were grown in 24-well plates treated with varying concentrations of heparin; cells were then collected and counted from day 1 to day 7. The total live cell was examined by the cell hemacytometer.

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Steps to reproduce

Two SKBR3 and MDA-MB-453 cells were obtained from ATCC. For the hallmark gene expression profiles, cells were plated in T75 flasks until 80% confluences. The gene expression was taken by Q-PCR. Gene expression was normalised against the 18S gene expression. For the cell proliferation data post-adding heparin cultures, cells were plated in 24-well plates and incubated at 37 °C in a 5% CO2 humidified atmosphere. Cells were harvested and 10 µL of cell suspension was taken for cell counting using the Trypan Blue exclusion method from day 1 to day 7. The cell number was presented as cell number x 1e4

Institutions

Queensland University of Technology

Categories

Breast Cancer, Gene Expression, HER2-Positive Breast Cancer

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