Raw and analyzed data of histological alterations and biochemical markers in rat kidney exposed to Cadmium and treated with Atorvastatin
The data set supports studies involving the protective effect of antioxidants against environmental and occupational cadmium contaminant, inducing oxidative kidney damage in rat model. Details of raw data and Tukey’s multiple comparison tests related to biochemical parameters (BUN, creatinine, GSH, MDA, SOD, and GPx) in the rat groups treated with saline, Atorvastatin (AT) and cadmium. The insights gained from these data may allow researchers to further explain the role of Atorvastatin antioxidant treatment on the cadmium chloride- induced oxidative stress in rat nephrotoxicity. These data indicate Atorvastatin improves lipid peroxidation, BUN, and Creatinine serum levels and reduces adverse histological changes in rat kidney tissues induced by cadmium chloride.
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Biochemical data were measured using Rat GPX ELISA Kit (Cat No: ZB-GPX-96A), Rat SOD ELISA Kit (Cat No: ZB-SOD-96A), Rat GSH ELISA Kit (Cat No: ZB-GSH-96A), and Rat MDA ELISA Kit (Cat No:ZB-MDA-96A). ELISA kits obtained from Zellbio Germany. BUN and CR in the kidney tissues were measured by Kits obtained from Pars Azmun Pharmaceutical, Tehran, Iran. Parameters determined were serum levels of BUN and CR and renal tissues concentrations of MDA, GSA, GPx, and SOD. Histological parameters measured for kidney tissue injury were cell nuclear dilation, loss of staining capacity and cellular swelling. Nephrotoxicity was induced in Wistar rat by the administration of cadmium chloride. Atorvastatin was administered for fifteen days. Histology of the kidney tissue and biochemical data were collected to evaluate the effect of Atorvastatin on these parameters in cadmium-induced nephrotoxicity. During each animal examination, the blood was collected from the heart of rats, which was subsequently centrifuged to obtain the serum. Kidneys were dissected, blocked, and for each tissue a 10 μm segment was sectioned and collected. The slides were made and dried and then stained with haematoxylin and eosin (H&E) protocols for histological analysis. Lastly, In the surface of each slide, five fields were randomly selected and evaluated under 400 x magnification by one pathologist and histologist.